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Sep. 10, 2014:
A total of 174 experimentally verified S-nitrosylation sites on 94 S-nitrosylated proteins from individualized human colorectal cancer tissues using a label-free quantitation strategy.

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Protein Name: Delta-1-pyrroline-5-carboxylate dehydrogenase, mitochondrial

UniprotKB/SwissProt ID: AL4A1_MOUSE (Q8CHT0)

Gene Name: Aldh4a1

Organism: Mus musculus (Mouse).

Function: Irreversible conversion of delta-1-pyrroline-5- carboxylate (P5C), derived either from proline or ornithine, to glutamate. This is a necessary step in the pathway interconnecting the urea and tricarboxylic acid cycles. The preferred substrate is glutamic gamma-semialdehyde, other substrates include succinic, glutaric and adipic semialdehydes (By similarity).

Other Modifications: View all modification sites in dbPTM

Protein Subcellular Localization: Mitochondrion matrix (By similarity).

PDB :
( If your security settings prevent Jmol from running, please register http://140.138.144.145/ as a safe location in your Java settings. )

Network with metabolic pathway:
Kegg map ID Pathway Link
map00250"Alanine, aspartate and glutamate metabolism"
map00330Arginine and proline metabolism
Graphical Visualization of S-nitrosylation Sites:
Overview of Protein S-nitrosylation Sites with Functional and Structural Information
InterPro ID Domain
IPR005931
IPR015590

3D Structure Databases:
3D structure databases
EntryMethodResolution (A)ChainPositionsView
3V9J X-ray 1.30 A A/B21-562Link
3V9K X-ray 1.50 A A/B21-562Link
3V9L X-ray 1.50 A A/B21-562Link
4E3X X-ray 1.24 A A/B21-562Link
4LGZ X-ray 1.68 A A/B21-562Link
4LH0 X-ray 1.67 A A/B21-562Link
4LH1 X-ray 1.67 A A/B21-562Link
4LH2 X-ray 1.67 A A/B21-562Link
4LH3 X-ray 1.81 A A/B21-562Link

The S-nitrosylation sites of AL4A1_MOUSE

No. Position S-nitrosylated Peptide Secondary Structure of S-nitrosylated Peptide Solvent Accessibility of nitrosylated Site Substrate Motifs PubMed ID Experiment
194FNHAHKVAKF C YADKALLNRA CCCCCEEEEE E CCCHHHHHHH 2.42%MC0322178444-
294FNHAHKVAKF C YADKALLNRA CCCCCEEEEE E CCCHHHHHHH 2.42%MC0320837516in vivo
3279GDTVTSSEHL C GINFTGSVPT HHHHHCCCCC C EEEECCCHHH 5.77%MC0921278135in vitro
4314FRTFPRLAGE C GGKNFHFVHS CCCCCEEEEE E CCCCCEEECC 9.48%MC0222178444-
5314FRTFPRLAGE C GGKNFHFVHS CCCCCEEEEE E CCCCCEEECC 9.48%MC0220837516in vivo
6314FRTFPRLAGE C GGKNFHFVHS CCCCCEEEEE E CCCCCEEECC 9.48%MC0222865876in vivo
7347RSAFEYGGQK C SACSRLYVPK HHHHCCCCCE E CCCCEEEECH 2.10%MC0422865876in vivo
8350FEYGGQKCSA C SRLYVPKSLW HCCCCCEECC C CEEEECHHHH 1.08%MC0522865876in vivo
9420PSLSILAGGQ C NESVGYYVEP CCCEEEECCC C CCCCCCEECC 7.44%MC1322865876in vivo
10431NESVGYYVEP C IIESKDPQEP CCCCCCEECC E EEECCCCCCH 2.87%MC0422865876in vivo