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Sep. 10, 2014:
A total of 174 experimentally verified S-nitrosylation sites on 94 S-nitrosylated proteins from individualized human colorectal cancer tissues using a label-free quantitation strategy.

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Protein Name: Glyceraldehyde-3-phosphate dehydrogenase

UniprotKB/SwissProt ID: G3P_RAT (P04797)

Gene Name: Gapdh

Synonyms: Gapd

Organism: Rattus norvegicus (Rat).

Function: Has both glyceraldehyde-3-phosphate dehydrogenase and nitrosylase activities, thereby playing a role in glycolysis and nuclear functions, respectively. Glyceraldehyde-3-phosphate dehydrogenase is a key enzyme in glycolysis that catalyzes the first step of the pathway by converting D-glyceraldehyde 3- phosphate (G3P) into 3-phospho-D-glyceroyl phosphate. Modulates the organization and assembly of the cytoskeleton. Facilitates the CHP1-dependent microtubule and membrane associations through its ability to stimulate the binding of CHP1 to microtubules. Also participates in nuclear events including transcription, RNA transport, DNA replication and apoptosis. Nuclear functions are probably due to the nitrosylase activity that mediates cysteine S- nitrosylation of nuclear target proteins such as SIRT1, HDAC2 and PRKDC. Component of the GAIT (gamma interferon-activated inhibitor of translation) complex which mediates interferon-gamma-induced transcript-selective translation inhibition in inflammation processes. Upon interferon-gamma treatment assembles into the GAIT complex which binds to stem loop-containing GAIT elements in the 3'-UTR of diverse inflammatory mRNAs (such as ceruplasmin) and suppresses their translation (By similarity).

Other Modifications: View all modification sites in dbPTM

Protein Subcellular Localization: Cytoplasm, cytosol. Cytoplasm, cytoskeleton. Nucleus. Note=Translocates to the nucleus following S- nitrosylation and interaction with SIAH1, which contains a nuclear localization signal. Colocalizes with CHP1 to small punctate structures along the micro

Network with metabolic pathway:
Kegg map ID Pathway Link
map00010Glycolysis / Gluconeogenesis
map04066HIF-1 signaling pathway
map05010Alzheimer's disease
Graphical Visualization of S-nitrosylation Sites:
Overview of Protein S-nitrosylation Sites with Functional and Structural Information
InterPro ID Domain
IPR000173
IPR006424

The S-nitrosylation sites of G3P_RAT

No. Position S-nitrosylated Peptide Secondary Structure of S-nitrosylated Peptide Solvent Accessibility of nitrosylated Site Substrate Motifs PubMed ID Experiment
123GRLVTRAAFS C DKVDIVAIND HHHHHHHHHH C CCCEEEEEEC 4.79%RC0417629318in vitro
2150NSLKIVSNAS C TTNCLAPLAK CCCCEEECCC C CHHHHHHHHH 5.17%RC047902582in vitro
3150NSLKIVSNAS C TTNCLAPLAK CCCCEEECCC C CHHHHHHHHH 5.17%RC048626764in vitro
4150NSLKIVSNAS C TTNCLAPLAK CCCCEEECCC C CHHHHHHHHH 5.17%RC0415951807-
5150NSLKIVSNAS C TTNCLAPLAK CCCCEEECCC C CHHHHHHHHH 5.17%RC0416418269in vitro
6150NSLKIVSNAS C TTNCLAPLAK CCCCEEECCC C CHHHHHHHHH 5.17%RC0418335467-
7150NSLKIVSNAS C TTNCLAPLAK CCCCEEECCC C CHHHHHHHHH 5.17%RC0419366988-
8154IVSNASCTTN C LAPLAKVIHD EEECCCCCHH H HHHHHHHHHH 3.01%RC0416418269in vitro
9154IVSNASCTTN C LAPLAKVIHD EEECCCCCHH H HHHHHHHHHH 3.01%RC0418335467-
10154IVSNASCTTN C LAPLAKVIHD EEECCCCCHH H HHHHHHHHHH 3.01%RC0419366988-
11245TPNVSVVDLT C RLEKPAKYDD CCCCEEEEEE E EECCCCCHHH 6.42%RC0216418269in vitro
12245TPNVSVVDLT C RLEKPAKYDD CCCCEEEEEE E EECCCCCHHH 6.42%RC0217629318in vitro
13245TPNVSVVDLT C RLEKPAKYDD CCCCEEEEEE E EECCCCCHHH 6.42%RC0218335467-
14245TPNVSVVDLT C RLEKPAKYDD CCCCEEEEEE E EECCCCCHHH 6.42%RC0219366988-