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Sep. 10, 2014:
A total of 174 experimentally verified S-nitrosylation sites on 94 S-nitrosylated proteins from individualized human colorectal cancer tissues using a label-free quantitation strategy.

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Protein Name: Hypoxia-inducible factor 1-alpha

UniprotKB/SwissProt ID: HIF1A_HUMAN (Q16665)

Gene Name: HIF1A

Synonyms: BHLHE78, MOP1, PASD8

Organism: Homo sapiens (Human).

Function: Functions as a master transcriptional regulator of the adaptive response to hypoxia. Under hypoxic conditions, activates the transcription of over 40 genes, including erythropoietin, glucose transporters, glycolytic enzymes, vascular endothelial growth factor, HILPDA, and other genes whose protein products increase oxygen delivery or facilitate metabolic adaptation to hypoxia. Plays an essential role in embryonic vascularization, tumor angiogenesis and pathophysiology of ischemic disease. Binds to core DNA sequence 5'-[AG]CGTG-3' within the hypoxia response element (HRE) of target gene promoters. Activation requires recruitment of transcriptional coactivators such as CREBPB and EP300. Activity is enhanced by interaction with both, NCOA1 or NCOA2. Interaction with redox regulatory protein APEX seems to activate CTAD and potentiates activation by NCOA1 and CREBBP. Involved in the axonal distribution and transport of mitochondria in neurons during hypoxia.

Other Modifications: View all modification sites in dbPTM

Protein Subcellular Localization: Cytoplasm. Nucleus. Note=Cytoplasmic in normoxia, nuclear translocation in response to hypoxia. Colocalizes with SUMO1 in the nucleus, under hypoxia.

PDB :
( If your security settings prevent Jmol from running, please register http://140.138.144.145/ as a safe location in your Java settings. )

Network with metabolic pathway:
Kegg map ID Pathway Link
map04066HIF-1 signaling pathway
map05211Renal cell carcinoma
map04150mTOR signaling pathway
map05200Pathways in cancer
Graphical Visualization of S-nitrosylation Sites:
Overview of Protein S-nitrosylation Sites with Functional and Structural Information
InterPro ID Domain
IPR000014
IPR000700
IPR001092
IPR001321
IPR001610
IPR011598
IPR013655
IPR014887

3D Structure Databases:
3D structure databases
EntryMethodResolution (A)ChainPositionsView
1D7G Model - D15-73Link
1H2K X-ray 2.15 A S786-826Link
1H2L X-ray 2.25 A S786-826Link
1H2M X-ray 2.50 A S775-826Link
1L3E NMR - A786-826Link
1L8C NMR - B776-826Link
1LM8 X-ray 1.85 A H556-575Link
1LQB X-ray 2.00 A D549-582Link
2ILM X-ray 2.30 A S786-826Link
3HQR X-ray 2.00 A S558-574Link
3HQU X-ray 2.30 A S558-574Link
4AJY X-ray 1.73 A H562-577Link
4H6J X-ray 1.52 A A238-348Link

The S-nitrosylation sites of HIF1A_HUMAN

No. Position S-nitrosylated Peptide Secondary Structure of S-nitrosylated Peptide Solvent Accessibility of nitrosylated Site Substrate Motifs PubMed ID Experiment
1520SPEPNSPSEY C FYVDSDMVNE CCCCCCCCCC C CCCCCCCCCC 1.42%HC1222178444-
2520SPEPNSPSEY C FYVDSDMVNE CCCCCCCCCC C CCCCCCCCCC 1.42%HC1217434127-
3800SGLPQLTSYD C EVNAPIQGSR HCCCCEECCC C EEECCCCCCC 4.83%HC1222178444-
4800SGLPQLTSYD C EVNAPIQGSR HCCCCEECCC C EEECCCCCCC 4.83%HC1215023551-
5800SGLPQLTSYD C EVNAPIQGSR HCCCCEECCC C EEECCCCCCC 4.83%HC1215688001-