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Sep. 10, 2014:
A total of 174 experimentally verified S-nitrosylation sites on 94 S-nitrosylated proteins from individualized human colorectal cancer tissues using a label-free quantitation strategy.

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Protein Name: cAMP-dependent protein kinase catalytic subunit alpha

UniprotKB/SwissProt ID: KAPCA_MOUSE (P05132)

Gene Name: Prkaca

Synonyms: Pkaca

Organism: Mus musculus (Mouse).

Function: Phosphorylates a large number of substrates in the cytoplasm and the nucleus. Regulates the abundance of compartmentalized pools of its regulatory subunits through phosphorylation of PJA2 which binds and ubiquitinates these subunits, leading to their subsequent proteolysis. Phosphorylates CDC25B, ABL1, NFKB1, CLDN3, PSMC5/RPT6, PJA2, RYR2, RORA and VASP. RORA is activated by phosphorylation. Required for glucose- mediated adipogenic differentiation increase and osteogenic differentiation inhibition from osteoblasts. Involved in the regulation of platelets in response to thrombin and collagen; maintains circulating platelets in a resting state by phosphorylating proteins in numerous platelet inhibitory pathways when in complex with NF-kappa-B (NFKB1 and NFKB2) and I-kappa-B- alpha (NFKBIA), but thrombin and collagen disrupt these complexes and free active PRKACA stimulates platelets and leads to platelet aggregation by phosphorylating VASP. Prevents the antiproliferative and anti-invasive effects of alpha- difluoromethylornithine in breast cancer cells when activated. RYR2 channel activity is potentiated by phosphorylation in presence of luminal Ca(2+), leading to reduced amplitude and increased frequency of store overload-induced Ca(2+) release (SOICR) characterized by an increased rate of Ca(2+) release and propagation velocity of spontaneous Ca(2+) waves, despite reduced wave amplitude and resting cytosolic Ca(2+). PSMC5/RPT6 activation by phosphorylation stimulates proteasome. Negatively regulates tight junctions (TJs) in ovarian cancer cells via CLDN3 phosphorylation. NFKB1 phosphorylation promotes NF-kappa-B p50-p50 DNA binding. Involved in embryonic development by down-regulating the Hedgehog (Hh) signaling pathway that determines embryo pattern formation and morphogenesis. Prevents meiosis resumption in prophase-arrested oocytes via CDC25B inactivation by phosphorylation. May also regulate rapid eye movement (REM) sleep in the pedunculopontine tegmental (PPT). Phosphorylates APOBEC3G and AICDA. Isoform 2 phosphorylates and activates ABL1 in sperm flagellum to promote spermatozoa capacitation (By similarity).

Other Modifications: View all modification sites in dbPTM

Protein Subcellular Localization: Cytoplasm. Cell membrane (By similarity). Nucleus (By similarity). Mitochondrion. Note=Translocates into the nucleus (monomeric catalytic subunit) (By similarity). The inactive holoenzyme is found in the cytoplasm. Distributed throughout the cytoplasm in

PDB :
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Network with metabolic pathway:
Kegg map ID Pathway Link
map04720Long-term potentiation
map04740Olfactory transduction
map04742Taste transduction
map04961Endocrine and other factor-regulated calcium reabsorption
map04962Vasopressin-regulated water reabsorption
map05020Prion diseases
map05030Cocaine addiction
map04210Apoptosis
map04340Hedgehog signaling pathway
map04540Gap junction
map04723Retrograde endocannabinoid signaling
map04724Glutamatergic synapse
map04725Cholinergic synapse
map04726Serotonergic synapse
map04727GABAergic synapse
map04728Dopaminergic synapse
map04912GnRH signaling pathway
map04970Salivary secretion
map04971Gastric acid secretion
map04976Bile secretion
map05031Amphetamine addiction
map05032Morphine addiction
map05146Amoebiasis
map05414Dilated cardiomyopathy
map04010MAPK signaling pathway
map04020Calcium signaling pathway
map04062Chemokine signaling pathway
map04114Oocyte meiosis
map04270Vascular smooth muscle contraction
map04310Wnt signaling pathway
map04910Insulin signaling pathway
map04914Progesterone-mediated oocyte maturation
map04916Melanogenesis
map05034Alcoholism
map05166HTLV-I infection
map05169Epstein-Barr virus infection
map05203Viral carcinogenesis
Graphical Visualization of S-nitrosylation Sites:
Overview of Protein S-nitrosylation Sites with Functional and Structural Information
InterPro ID Domain
IPR000719
IPR000961
IPR002290
IPR008271
IPR011009

The S-nitrosylation sites of KAPCA_MOUSE

No. Position S-nitrosylated Peptide Secondary Structure of S-nitrosylated Peptide Solvent Accessibility of nitrosylated Site Substrate Motifs PubMed ID Experiment
1200KRVKGRTWTL C GTPEYLAPEI EEECCCCEEE E ECHHHCCHHH 4.45%MC0122865876in vivo