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Sep. 10, 2014:
A total of 174 experimentally verified S-nitrosylation sites on 94 S-nitrosylated proteins from individualized human colorectal cancer tissues using a label-free quantitation strategy.

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Protein Name: Mitogen-activated protein kinase 1

UniprotKB/SwissProt ID: MK01_MOUSE (P63085)

Gene Name: Mapk1

Synonyms: Erk2, Mapk, Prkm1

Organism: Mus musculus (Mouse).

Function: Serine/threonine kinase which acts as an essential component of the MAP kinase signal transduction pathway. MAPK1/ERK2 and MAPK3/ERK1 are the 2 MAPKs which play an important role in the MAPK/ERK cascade. They participate also in a signaling cascade initiated by activated KIT and KITLG/SCF. Depending on the cellular context, the MAPK/ERK cascade mediates diverse biological functions such as cell growth, adhesion, survival and differentiation through the regulation of transcription, translation, cytoskeletal rearrangements. The MAPK/ERK cascade plays also a role in initiation and regulation of meiosis, mitosis, and postmitotic functions in differentiated cells by phosphorylating a number of transcription factors. About 160 substrates have already been discovered for ERKs. Many of these substrates are localized in the nucleus, and seem to participate in the regulation of transcription upon stimulation. However, other substrates are found in the cytosol as well as in other cellular organelles, and those are responsible for processes such as translation, mitosis and apoptosis. Moreover, the MAPK/ERK cascade is also involved in the regulation of the endosomal dynamics, including lysosome processing and endosome cycling through the perinuclear recycling compartment (PNRC); as well as in the fragmentation of the Golgi apparatus during mitosis. The substrates include transcription factors (such as ATF2, BCL6, ELK1, ERF, FOS, HSF4 or SPZ1), cytoskeletal elements (such as CANX, CTTN, GJA1, MAP2, MAPT, PXN, SORBS3 or STMN1), regulators of apoptosis (such as BAD, BTG2, CASP9, DAPK1, IER3, MCL1 or PPARG), regulators of translation (such as EIF4EBP1) and a variety of other signaling-related molecules (like ARHGEF2, DCC, FRS2 or GRB10). Protein kinases (such as RAF1, RPS6KA1/RSK1, RPS6KA3/RSK2, RPS6KA2/RSK3, RPS6KA6/RSK4, SYK, MKNK1/MNK1, MKNK2/MNK2, RPS6KA5/MSK1, RPS6KA4/MSK2, MAPKAPK3 or MAPKAPK5) and phosphatases (such as DUSP1, DUSP4, DUSP6 or DUSP16) are other substrates which enable the propagation the MAPK/ERK signal to additional cytosolic and nuclear targets, thereby extending the specificity of the cascade. Mediates phosphorylation of TPR in respons to EGF stimulation. May play a role in the spindle assembly checkpoint. Phosphorylates PML and promotes its interaction with PIN1, leading to PML degradation (By similarity). Acts as a transcriptional repressor. Binds to a [GC]AAA[GC] consensus sequence. Repress the expression of interferon gamma-induced genes. Seems to bind to the promoter of CCL5, DMP1, IFIH1, IFITM1, IRF7, IRF9, LAMP3, OAS1, OAS2, OAS3 and STAT1. Transcriptional activity is independent of kinase activity (By similarity).

Other Modifications: View all modification sites in dbPTM

Protein Subcellular Localization: Cytoplasm, cytoskeleton, spindle (By similarity). Nucleus. Cytoplasm, cytoskeleton, microtubule organizing center, centrosome (By similarity). Cytoplasm. Note=Associated with the spindle during prometaphase and metaphase (By similarity). PEA15-binding an

Network with metabolic pathway:
Kegg map ID Pathway Link
map04320Dorso-ventral axis formation
map04664Fc epsilon RI signaling pathway
map04720Long-term potentiation
map04930Type II diabetes mellitus
map04960Aldosterone-regulated sodium reabsorption
map05020Prion diseases
map05213Endometrial cancer
map05216Thyroid cancer
map05219Bladder cancer
map05221Acute myeloid leukemia
map05223Non-small cell lung cancer
map04066HIF-1 signaling pathway
map04360Axon guidance
map04370VEGF signaling pathway
map04380Osteoclast differentiation
map04520Adherens junction
map04540Gap junction
map04621NOD-like receptor signaling pathway
map04650Natural killer cell mediated cytotoxicity
map04660T cell receptor signaling pathway
map04662B cell receptor signaling pathway
map04666Fc gamma R-mediated phagocytosis
map04722Neurotrophin signaling pathway
map04723Retrograde endocannabinoid signaling
map04724Glutamatergic synapse
map04725Cholinergic synapse
map04726Serotonergic synapse
map04730Long-term depression
map04912GnRH signaling pathway
map05133Pertussis
map05140Leishmaniasis
map05142Chagas disease (American trypanosomiasis)
map05145Toxoplasmosis
map05160Hepatitis C
map05210Colorectal cancer
map05211Renal cell carcinoma
map05212Pancreatic cancer
map05214Glioma
map05215Prostate cancer
map05218Melanoma
map05220Chronic myeloid leukemia
map04010MAPK signaling pathway
map04012ErbB signaling pathway
map04062Chemokine signaling pathway
map04114Oocyte meiosis
map04150mTOR signaling pathway
map04151PI3K-Akt signaling pathway
map04270Vascular smooth muscle contraction
map04350TGF-beta signaling pathway
map04510Focal adhesion
map04620Toll-like receptor signaling pathway
map04810Regulation of actin cytoskeleton
map04910Insulin signaling pathway
map04914Progesterone-mediated oocyte maturation
map04916Melanogenesis
map05010Alzheimer's disease
map05034Alcoholism
map05132Salmonella infection
map05152Tuberculosis
map05161Hepatitis B
map05164Influenza A
map05200Pathways in cancer
map05203Viral carcinogenesis
Graphical Visualization of S-nitrosylation Sites:
Overview of Protein S-nitrosylation Sites with Functional and Structural Information
InterPro ID Domain
IPR000719
IPR002290
IPR003527
IPR008271
IPR008349
IPR011009

The S-nitrosylation sites of MK01_MOUSE

No. Position S-nitrosylated Peptide Secondary Structure of S-nitrosylated Peptide Solvent Accessibility of nitrosylated Site Substrate Motifs PubMed ID Experiment
163KISPFEHQTY C QRTLREIKIL EECCCCCCCH H HHHHHHHHHH 2.21%MC0420925432in vivo
2159KPSNLLLNTT C DLKICDFGLA CCCCEEECCC C CEEEEECCEE 5.62%MC1222178444-
3159KPSNLLLNTT C DLKICDFGLA CCCCEEECCC C CEEEEECCEE 5.62%MC1219101475-
4159KPSNLLLNTT C DLKICDFGLA CCCCEEECCC C CEEEEECCEE 5.62%MC1219483679in vivo