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Sep. 10, 2014:
A total of 174 experimentally verified S-nitrosylation sites on 94 S-nitrosylated proteins from individualized human colorectal cancer tissues using a label-free quantitation strategy.

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Protein Name: NADH-ubiquinone oxidoreductase 75 kDa subunit, mitochondrial

UniprotKB/SwissProt ID: NDUS1_MOUSE (Q91VD9)

Gene Name: Ndufs1

Organism: Mus musculus (Mouse).

Function: Core subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I) that is believed to belong to the minimal assembly required for catalysis. Complex I functions in the transfer of electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone (By similarity). This is the largest subunit of complex I and it is a component of the iron-sulfur (IP) fragment of the enzyme. It may form part of the active site crevice where NADH is oxidized (By similarity).

Other Modifications: View all modification sites in dbPTM

Protein Subcellular Localization: Mitochondrion inner membrane (By similarity). Note=Matrix and cytoplasmic side of the mitochondrial inner membrane (By similarity).

Network with metabolic pathway:
Kegg map ID Pathway Link
map00190Oxidative phosphorylation
map05010Alzheimer's disease
map05012Parkinson's disease
map05016Huntington's disease
Graphical Visualization of S-nitrosylation Sites:
Overview of Protein S-nitrosylation Sites with Functional and Structural Information
InterPro ID Domain
IPR000283
IPR001041
IPR006058
IPR006656
IPR010228
IPR015405

The S-nitrosylation sites of NDUS1_MOUSE

No. Position S-nitrosylated Peptide Secondary Structure of S-nitrosylated Peptide Solvent Accessibility of nitrosylated Site Substrate Motifs PubMed ID Experiment
164EKVGMQIPRF C YHERLSVAGN HHHCCCCCCH H CHHCCCCCCC 3.41%MC0621278135in vitro
275YHERLSVAGN C RMCLVEIEKA CHHCCCCCCC C CEEEEEECCC 2.61%MC0121278135in vitro
378RLSVAGNCRM C LVEIEKAPKV CCCCCCCCCE E EEEECCCCCE 1.67%MC0222865876in vivo
492IEKAPKVVAA C AMPVMKGWNI ECCCCCEEEE E EEECCCCCEE 2.10%MC0221278135in vitro
592IEKAPKVVAA C AMPVMKGWNI ECCCCCEEEE E EEECCCCCEE 2.10%MC0222865876in vivo
6367LLNKVDSDNL C TEEIFPTEGA HHHHHCCCCC C CCCCCHHHHH 5.88%MC0121278135in vitro
7463QDIASGRHSF C EVLKDAKKPM HHHHHHHHHH H HHHHHCCCCE 3.86%MC0521278135in vitro
8554MLFLLGADGG C ITRQDLPKDC EEEEECCCCC C CCHHHHCCCC 3.34%MC1221278135in vitro
9554MLFLLGADGG C ITRQDLPKDC EEEEECCCCC C CCHHHHCCCC 3.34%MC1222865876in vivo
10564CITRQDLPKD C FIVYQGHHGD CCCHHHHCCC C EEEEECCCCC 2.90%MC1121278135in vitro
11710ISRASQTMAK C VKAVTEGAQA HHHHHHHHHH H HHHHHHHHHH 2.49%MC0621278135in vitro
12727GAQAVEEPSI C ---------- HHHHHCCCCC C  5.53%MC1321278135in vitro
13727GAQAVEEPSI C ---------- HHHHHCCCCC C  5.53%MC1322865876in vivo