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Sep. 10, 2014:
A total of 174 experimentally verified S-nitrosylation sites on 94 S-nitrosylated proteins from individualized human colorectal cancer tissues using a label-free quantitation strategy.

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Protein Name: Non-POU domain-containing octamer-binding protein

UniprotKB/SwissProt ID: NONO_MOUSE (Q99K48)

Gene Name: Nono

Organism: Mus musculus (Mouse).

Function: DNA- and RNA binding protein, involved in several nuclear processes. Binds the conventional octamer sequence in double-stranded DNA. Also binds single-stranded DNA and RNA at a site independent of the duplex site. Involved in pre-mRNA splicing, probably as a heterodimer with SFPQ. Interacts with U5 snRNA, probably by binding to a purine-rich sequence located on the 3' side of U5 snRNA stem 1b. Together with PSPC1, required for the formation of nuclear paraspeckles. The SFPQ-NONO heteromer associated with MATR3 may play a role in nuclear retention of defective RNAs. The SFPQ-NONO heteromer may be involved in DNA unwinding by modulating the function of topoisomerase I/TOP1. The SFPQ-NONO heteromer may be involved in DNA nonhomologous end joining (NHEJ) required for double-strand break repair and V(D)J recombination and may stabilize paired DNA ends. In vitro, the complex strongly stimulates DNA end joining, binds directly to the DNA substrates and cooperates with the Ku70/G22P1-Ku80/XRCC5 (Ku) dimer to establish a functional preligation complex. NONO is involved in transcriptional regulation. The SFPQ-NONO-NR5A1 complex binds to the CYP17 promoter and regulates basal and cAMP- dependent transcriptional avtivity (By similarity). NONO binds to an enhancer element in long terminal repeats of endogenous intracisternal A particles (IAPs) and activates transcription.

Other Modifications: View all modification sites in dbPTM

Protein Subcellular Localization: Nucleus (By similarity). Nucleus, nucleolus (By similarity). Nucleus speckle (By similarity). Note=Detected in punctate subnuclear structures often located adjacent to splicing speckles, called paraspeckles (By similarity).

PDB :
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Graphical Visualization of S-nitrosylation Sites:
Overview of Protein S-nitrosylation Sites with Functional and Structural Information
InterPro ID Domain
IPR000504
IPR012677
IPR012975

3D Structure Databases:
3D structure databases
EntryMethodResolution (A)ChainPositionsView
2CPJ NMR - A68-153Link
2RS8 NMR - A68-153Link

The S-nitrosylation sites of NONO_MOUSE

No. Position S-nitrosylated Peptide Secondary Structure of S-nitrosylated Peptide Solvent Accessibility of nitrosylated Site Substrate Motifs PubMed ID Experiment
1147RGKQLRVRFA C HSASLTVRNL CCEEEEEEEC C CCCCEEECCC 2.10%MC1320925432in vivo
2147RGKQLRVRFA C HSASLTVRNL CCEEEEEEEC C CCCCEEECCC 2.10%MC1321278135in vitro