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Sep. 10, 2014:
A total of 174 experimentally verified S-nitrosylation sites on 94 S-nitrosylated proteins from individualized human colorectal cancer tissues using a label-free quantitation strategy.

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Protein Name: Pyruvate carboxylase, mitochondrial

UniprotKB/SwissProt ID: PYC_MOUSE (Q05920)

Gene Name: Pc

Synonyms: Pcx

Organism: Mus musculus (Mouse).

Function: Pyruvate carboxylase catalyzes a 2-step reaction, involving the ATP-dependent carboxylation of the covalently attached biotin in the first step and the transfer of the carboxyl group to pyruvate in the second. Catalyzes in a tissue specific manner, the initial reactions of glucose (liver, kidney) and lipid (adipose tissue, liver, brain) synthesis from pyruvate.

Other Modifications: View all modification sites in dbPTM

Protein Subcellular Localization: Mitochondrion matrix.

Graphical Visualization of S-nitrosylation Sites:
Overview of Protein S-nitrosylation Sites with Functional and Structural Information
InterPro ID Domain
IPR000089
IPR000891
IPR001882
IPR003379
IPR005479
IPR005481
IPR005482
IPR005930
IPR011053
IPR011054
IPR011761
IPR011764
IPR013785
IPR013816
IPR013817

The S-nitrosylation sites of PYC_MOUSE

No. Position S-nitrosylated Peptide Secondary Structure of S-nitrosylated Peptide Solvent Accessibility of nitrosylated Site Substrate Motifs PubMed ID Experiment
1131LSERADFAQA C QDAGVRFIGP CCCCCHHHHH H HHCCCCEECC 2.21%MC0122178444-
2131LSERADFAQA C QDAGVRFIGP CCCCCHHHHH H HHCCCCEECC 2.21%MC0120837516in vivo
3663NYPDNVVFKF C EVAKENGMDV CCCHHHHHHH H HHHHHCCCCE 4.19%MC0722178444-
4663NYPDNVVFKF C EVAKENGMDV CCCHHHHHHH H HHHHHCCCCE 4.19%MC0720837516in vivo
5752DMAGLLKPAA C TMLVSSLRDR CEEECCCHHH H HHHHHHHHHH 2.20%MC1022178444-
6752DMAGLLKPAA C TMLVSSLRDR CEEECCCHHH H HHHHHHHHHH 2.20%MC1020837516in vivo