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Sep. 10, 2014:
A total of 174 experimentally verified S-nitrosylation sites on 94 S-nitrosylated proteins from individualized human colorectal cancer tissues using a label-free quantitation strategy.

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Protein Name: 30S ribosomal protein S12

UniprotKB/SwissProt ID: RS12_ECOLI (P0A7S3)

Gene Name: rpsL

Synonyms: strA

Organism: Escherichia coli (strain K12).

Function: With S4 and S5 plays an important role in translational accuracy. Interacts with and stabilizes bases of the 16S rRNA that are involved in tRNA selection in the A site and with the mRNA backbone. Located at the interface of the 30S and 50S subunits, it traverses the body of the 30S subunit contacting proteins on the other side and probably holding the rRNA structure together. The combined cluster of proteins S8, S12 and S17 appears to hold together the shoulder and platform of the 30S subunit (By similarity). Cryo-EM studies suggest that S12 contacts the EF-Tu bound tRNA in the A-site during codon-recognition. This contact is most likely broken as the aminoacyl-tRNA moves into the peptidyl transferase center in the 50S subunit.

Other Modifications: View all modification sites in dbPTM

Protein Subcellular Localization:

PDB :
( If your security settings prevent Jmol from running, please register http://140.138.144.145/ as a safe location in your Java settings. )

Graphical Visualization of S-nitrosylation Sites:
Overview of Protein S-nitrosylation Sites with Functional and Structural Information
InterPro ID Domain
IPR005679
IPR006032
IPR012340
3D Structure Databases:
3D structure databases
EntryMethodResolution (A)ChainPositionsView
1M5G Model - L2-124Link
1MJ1 EM 13.00 A O5-123Link
1P6G EM 12.30 A L2-124Link
1P87 EM 11.50 A L2-124Link
1VS5 X-ray 3.46 A L1-124Link
1VS7 X-ray 3.46 A L1-124Link
1ZN1 EM 14.10 A L28-123Link
2AVY X-ray 3.46 A L2-124Link
2AW7 X-ray 3.46 A L2-124Link
2GY9 EM 15.00 A L23-122Link
2GYB EM 15.00 A L23-122Link
2I2P X-ray 3.22 A L2-123Link
2I2U X-ray 3.22 A L2-123Link
2QAL X-ray 3.21 A L2-124Link
2QAN X-ray 3.21 A L2-124Link
2QB9 X-ray 3.54 A L2-124Link
2QBB X-ray 3.54 A L2-124Link
2QBD X-ray 3.30 A L2-124Link
2QBF X-ray 3.30 A L2-124Link
2QBH X-ray 4.00 A L2-124Link
2QBJ X-ray 4.00 A L2-124Link
2QOU X-ray 3.93 A L2-124Link
2QOW X-ray 3.93 A L2-124Link
2QOY X-ray 3.50 A L2-124Link
2QP0 X-ray 3.50 A L2-124Link
2VHO X-ray 3.74 A L2-124Link
2VHP X-ray 3.74 A L2-124Link
2WWL EM 5.80 A L2-124Link
2YKR EM 9.80 A L2-124Link
2Z4K X-ray 4.45 A L2-124Link
2Z4M X-ray 4.45 A L2-124Link
3DEG EM - D2-124Link
3DF1 X-ray 3.50 A L2-123Link
3DF3 X-ray 3.50 A L2-123Link
3E1A EM - D1-124Link
3E1C EM - D1-124Link
3EP2 EM - L2-124Link
3EQ3 EM - L2-124Link
3EQ4 EM - L2-124Link
3FIH EM 6.70 A L2-124Link
3I1M X-ray 3.19 A L1-124Link
3I1O X-ray 3.19 A L1-124Link
3I1Q X-ray 3.81 A L1-124Link
3I1S X-ray 3.81 A L1-124Link
3I1Z X-ray 3.71 A L1-124Link
3I21 X-ray 3.71 A L1-124Link
3IZV EM - P1-124Link
3IZW EM - P1-124Link
3J00 EM - L2-124Link
3J0D EM 11.10 A I2-124Link
3J0E EM 9.90 A F2-124Link
3J0U EM 12.10 A O2-124Link
3J0V EM 14.70 A O2-124Link
3J0X EM 13.50 A O2-124Link
3J0Z EM 11.50 A O2-124Link
3J10 EM 11.50 A O2-124Link
3J13 EM 13.10 A N2-124Link
3J18 EM 8.30 A L2-124Link
3J36 EM 9.80 A L2-124Link
3J4V EM 12.00 A L1-124Link
3J4W EM 12.00 A L1-124Link
3J4Y EM 17.00 A L1-124Link
3J4Z EM 20.00 A L1-124Link
3J53 EM 13.00 A L1-124Link
3J55 EM 15.00 A L1-124Link
3J57 EM 17.00 A L1-124Link
3J59 EM 12.00 A L1-124Link
3J5B EM 17.00 A L1-124Link
3J5D EM 17.00 A L1-124Link
3J5F EM 20.00 A L1-124Link
3J5H EM 15.00 A L1-124Link
3J5J EM 9.00 A L1-124Link
3J5N EM 6.80 A L1-124Link
3J5T EM 7.60 A L2-124Link
3J5X EM 7.60 A L2-124Link
3KC4 EM - L1-124Link
3OAQ X-ray 3.25 A L2-124Link
3OAR X-ray 3.25 A L2-124Link
3OFA X-ray 3.19 A L2-124Link
3OFB X-ray 3.19 A L2-124Link
3OFO X-ray 3.10 A L2-124Link
3OFP X-ray 3.10 A L2-124Link
3OFX X-ray 3.29 A L2-124Link
3OFY X-ray 3.29 A L2-124Link
3OR9 X-ray 3.30 A L1-124Link
3ORA X-ray 3.30 A L1-124Link
3SFS X-ray 3.20 A L1-124Link
3UOQ X-ray 3.70 A L1-124Link
4A2I EM 16.50 A L2-124Link
4ADV EM 13.50 A L2-124Link
4GAQ X-ray 3.30 A L1-124Link
4GAS X-ray 3.30 A L1-124Link
4GD1 X-ray 3.00 A L2-124Link
4GD2 X-ray 3.00 A L2-124Link
4KIY X-ray 2.90 A L1-124Link
4KJ0 X-ray 2.90 A L1-124Link
4KJ2 X-ray 2.90 A L1-124Link
4KJ4 X-ray 2.90 A L1-124Link
4KJ6 X-ray 2.90 A L1-124Link
4KJ8 X-ray 2.90 A L1-124Link
4KJA X-ray 2.90 A L1-124Link
4KJC X-ray 2.90 A L1-124Link
The S-nitrosylation sites of RS12_ECOLI

No. Position S-nitrosylated Peptide Secondary Structure of S-nitrosylated Peptide Solvent Accessibility of nitrosylated Site Substrate Motifs PubMed ID Experiment
127AKSNVPALEA C PQKRGVCTRV CCCCCCCCCC C CCCCEEEEEE 2.40%EC0222178444-
227AKSNVPALEA C PQKRGVCTRV CCCCCCCCCC C CCCCEEEEEE 2.40%EC0219483679in vitro