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Sep. 10, 2014:
A total of 174 experimentally verified S-nitrosylation sites on 94 S-nitrosylated proteins from individualized human colorectal cancer tissues using a label-free quantitation strategy.

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Protein Name: Matrix metalloproteinase-9

UniprotKB/SwissProt ID: MMP9_HUMAN (P14780)

Gene Name: MMP9

Synonyms: CLG4B

Organism: Homo sapiens (Human).

Function: May play an essential role in local proteolysis of the extracellular matrix and in leukocyte migration. Could play a role in bone osteoclastic resorption. Cleaves KiSS1 at a Gly-|-Leu bond. Cleaves type IV and type V collagen into large C-terminal three quarter fragments and shorter N-terminal one quarter fragments. Degrades fibronectin but not laminin or Pz-peptide.

Other Modifications: View all modification sites in dbPTM

Protein Subcellular Localization: Secreted, extracellular space, extracellular matrix (Probable).

PDB :
( If your security settings prevent Jmol from running, please register http://140.138.144.145/ as a safe location in your Java settings. )

Network with metabolic pathway:
Kegg map ID Pathway Link
map05219Bladder cancer
map04670Leukocyte transendothelial migration
map05161Hepatitis B
map05200Pathways in cancer
map05202Transcriptional misregulation in cancer
Graphical Visualization of S-nitrosylation Sites:
Overview of Protein S-nitrosylation Sites with Functional and Structural Information
InterPro ID Domain
IPR000562
IPR000585
IPR001818
IPR002477
IPR006025
IPR006026
IPR006970
IPR013806

3D Structure Databases:
3D structure databases
EntryMethodResolution (A)ChainPositionsView
1GKC X-ray 2.30 A A/B107-443Link
1GKD X-ray 2.10 A A/B107-443Link
1ITV X-ray 1.95 A A/B513-707Link
1L6J X-ray 2.50 A A20-444Link
1LKG Model - A1-707Link
2OVX X-ray 2.00 A A/B110-443Link
2OVZ X-ray 2.00 A A/B110-443Link
2OW0 X-ray 2.00 A A/B110-443Link
2OW1 X-ray 2.20 A A/B110-443Link
2OW2 X-ray 2.90 A A/B110-443Link
4H1Q X-ray 1.59 A A/B110-444Link
4H2E X-ray 2.90 A A/B107-444Link
4H3X X-ray 1.76 A A/B107-444Link
4H82 X-ray 1.90 A A/B/C/D110-444Link
4HMA X-ray 1.94 A A/B110-444Link

The S-nitrosylation sites of MMP9_HUMAN

No. Position S-nitrosylated Peptide Secondary Structure of S-nitrosylated Peptide Solvent Accessibility of nitrosylated Site Substrate Motifs PubMed ID Experiment
199ATLKAMRTPR C GVPDLGRFQT HHHHHHCCCC C CCCCCCCCCC 7.30%HC0122178444-
299ATLKAMRTPR C GVPDLGRFQT HHHHHHCCCC C CCCCCCCCCC 7.30%HC0112183632in vitro