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Sep. 10, 2014:
A total of 174 experimentally verified S-nitrosylation sites on 94 S-nitrosylated proteins from individualized human colorectal cancer tissues using a label-free quantitation strategy.

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Protein Name: Vesicle-fusing ATPase

UniprotKB/SwissProt ID: NSF_HUMAN (P46459)

Gene Name: NSF

Organism: Homo sapiens (Human).

Function: Required for vesicle-mediated transport. Catalyzes the fusion of transport vesicles within the Golgi cisternae. Is also required for transport from the endoplasmic reticulum to the Golgi stack. Seems to function as a fusion protein required for the delivery of cargo proteins to all compartments of the Golgi stack independent of vesicle origin. Interaction with AMPAR subunit GRIA2 leads to influence GRIA2 membrane cycling (By similarity).

Other Modifications: View all modification sites in dbPTM

Protein Subcellular Localization: Cytoplasm.

Network with metabolic pathway:
Kegg map ID Pathway Link
map04721Synaptic vesicle cycle
map04962Vasopressin-regulated water reabsorption
map04727GABAergic synapse
Graphical Visualization of S-nitrosylation Sites:
Overview of Protein S-nitrosylation Sites with Functional and Structural Information
InterPro ID Domain
IPR001984
IPR003338
IPR003593
IPR003959
IPR003960
IPR004201
IPR009010

The S-nitrosylation sites of NSF_HUMAN

No. Position S-nitrosylated Peptide Secondary Structure of S-nitrosylated Peptide Solvent Accessibility of nitrosylated Site Substrate Motifs PubMed ID Experiment
121CPTDELSLTN C AVVNEKDFQS CCCCCEEEEE E EEECCCCCCC 2.27%HC1222178444-
221CPTDELSLTN C AVVNEKDFQS CCCCCEEEEE E EEECCCCCCC 2.27%HC1214567912-
391SLYTFDKAKQ C IGTMTIEIDF EECCCCCCCC E EECCCEEEEE 2.82%HC0922178444-
491SLYTFDKAKQ C IGTMTIEIDF EECCCCCCCC E EECCCEEEEE 2.82%HC0914567912-
591SLYTFDKAKQ C IGTMTIEIDF EECCCCCCCC E EECCCEEEEE 2.82%HC0915944123-
6250FPPEIVEQMG C KHVKGILLYG CCHHHHHHCC C CCCCCEEEEC 2.01%HC0522178444-
7250FPPEIVEQMG C KHVKGILLYG CCHHHHHHCC C CCCCCEEEEC 2.01%HC0516418269in vitro
8264KGILLYGPPG C GKTLLARQIG CCEEEECCCC C CHHHHHHHHH 6.61%HC0522178444-
9264KGILLYGPPG C GKTLLARQIG CCEEEECCCC C CHHHHHHHHH 6.61%HC0524105792-
10264KGILLYGPPG C GKTLLARQIG CCEEEECCCC C CHHHHHHHHH 6.61%HC0514567912-