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Sep. 10, 2014:
A total of 174 experimentally verified S-nitrosylation sites on 94 S-nitrosylated proteins from individualized human colorectal cancer tissues using a label-free quantitation strategy.

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Protein Name: Phosphoenolpyruvate-protein phosphotransferase

UniprotKB/SwissProt ID: PT1_ECOLI (P08839)

Gene Name: ptsI

Organism: Escherichia coli (strain K12).

Function: General (non sugar-specific) component of the phosphoenolpyruvate-dependent sugar phosphotransferase system (sugar PTS). This major carbohydrate active-transport system catalyzes the phosphorylation of incoming sugar substrates concomitantly with their translocation across the cell membrane. Enzyme I transfers the phosphoryl group from phosphoenolpyruvate (PEP) to the phosphoryl carrier protein (HPr).

Other Modifications: View all modification sites in dbPTM

Protein Subcellular Localization: Cytoplasm.

PDB :
( If your security settings prevent Jmol from running, please register http://140.138.144.145/ as a safe location in your Java settings. )

Graphical Visualization of S-nitrosylation Sites:
Overview of Protein S-nitrosylation Sites with Functional and Structural Information
InterPro ID Domain
IPR000121
IPR006318
IPR008279
IPR008731
IPR015813

3D Structure Databases:
3D structure databases
EntryMethodResolution (A)ChainPositionsView
1EZA NMR - A1-258Link
1EZB NMR - A1-258Link
1EZC NMR - A1-258Link
1EZD NMR - A1-258Link
1ZYM X-ray 2.50 A A/B1-258Link
2EZA NMR - A1-258Link
2EZB NMR - A1-258Link
2EZC NMR - A1-258Link
2HWG X-ray 2.70 A A/B1-575Link
2KX9 Other - A/B1-573Link
2L5H Other - A/B1-573Link
2XDF Other - A/B1-573Link
3EZA NMR - A1-249Link
3EZB NMR - A1-258Link
3EZE NMR - A1-258Link

The S-nitrosylation sites of PT1_ECOLI

No. Position S-nitrosylated Peptide Secondary Structure of S-nitrosylated Peptide Solvent Accessibility of nitrosylated Site Substrate Motifs PubMed ID Experiment
1324FAAYKAVAEA C GSQAVIVRTM HHHHHHHHHH H CCCCEEEEEC 3.97%EC0222178444-
2324FAAYKAVAEA C GSQAVIVRTM HHHHHHHHHH H CCCCEEEEEC 3.97%EC0219483679in vitro