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Sep. 10, 2014:
A total of 174 experimentally verified S-nitrosylation sites on 94 S-nitrosylated proteins from individualized human colorectal cancer tissues using a label-free quantitation strategy.

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Protein Name: Adenylosuccinate synthetase isozyme 1

UniprotKB/SwissProt ID: PURA1_MOUSE (P28650)

Gene Name: Adssl1

Synonyms: Adss1

Organism: Mus musculus (Mouse).

Function: Component of the purine nucleotide cycle (PNC), which interconverts IMP and AMP to regulate the nucleotide levels in various tissues, and which contributes to glycolysis and ammoniagenesis. Catalyzes the first committed step in the biosynthesis of AMP from IMP.

Other Modifications: View all modification sites in dbPTM

Protein Subcellular Localization: Cytoplasm. Membrane; Peripheral membrane protein. Note=Partially associated with particulate fractions.

PDB :
( If your security settings prevent Jmol from running, please register http://140.138.144.145/ as a safe location in your Java settings. )

Network with metabolic pathway:
Kegg map ID Pathway Link
map00230Purine metabolism
map00250"Alanine, aspartate and glutamate metabolism"
Graphical Visualization of S-nitrosylation Sites:
Overview of Protein S-nitrosylation Sites with Functional and Structural Information
InterPro ID Domain
IPR001114

3D Structure Databases:
3D structure databases
EntryMethodResolution (A)ChainPositionsView
1IWE X-ray 2.10 A A/B1-457Link
1J4B X-ray 2.50 A A1-457Link
1LNY X-ray 2.20 A A/B1-457Link
1LON X-ray 2.10 A A1-457Link
1LOO X-ray 2.20 A A1-457Link
1MEZ X-ray 2.40 A A1-457Link
1MF0 X-ray 2.50 A A1-457Link
1MF1 X-ray 2.70 A A1-457Link
2DGN X-ray 2.40 A A1-457Link

The S-nitrosylation sites of PURA1_MOUSE

No. Position S-nitrosylated Peptide Secondary Structure of S-nitrosylated Peptide Solvent Accessibility of nitrosylated Site Substrate Motifs PubMed ID Experiment
163LATDADIVSR C QGGNNAGHTV ECCCCCEEEE C CCCCCCCEEE 3.25%MC1322865876in vivo
2183SKAARTGLRI C DLLSDFDEFS HHHHHHHHHH H HHHCCHHHHH 2.14%MC0821278135in vitro